Comparative toxicity of the nonsteroidal ecdysone agonists tebufenozide and methoxyfenozide to early and late larval instars of the whitemarked tussock moth, Orgyia leucostigma

Frédéric Dallaire, Michel Cusson

Abstract


Insecticidal toxicity can vary widely as a function of the developmental stage of the insect being targeted. Here the toxicity of the nonsteroidal ecdysone agonists tebufenozide and methoxyfenozide was evaluated against early and late instars of the white-marked tussock moth, Orgyia leucostigma (J.E. Smith) (Lepidoptera: Erebidae), using a droplet-feeding bioassay. On the basis of L LD50 estimates, methoxyfenozide was ~9 and 22 times more effective than tebufenozide in inducing mortality in 1st and 4th instars of O. leucostigma, respectively. U Unlike methoxyfenozide, tebufenozide was unable to cause more than 70% mortality in 4th instars, even at the highest dose tested (1 μg/larva). A Analysis of the present data suggests that susceptibility of late instar larvae to tebufenozide is significantly compromised whereas that to methoxyfenozide is much less so, which suggests that the mechanism conferring resistance to tebufenozide in late instar larvae is either ineffective or less effective in the case of methoxyfenozide.


Keywords


tebufenozide, methoxyfenoside, LD50, developmental variation in toxicity

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References


Bell, R R.A., Owens, C.D., Shapiro, M., and Tardif, J.R. 1981. Mass rearing and virus production. In Doane C.C., and McManus M.L. (eds) The Gypsy Moth: Research toward integrated pest management. U USDA AA AA APHIS Technical Bulletin 1584. Pp. 599–656.

Bliss, C.I. 1934. The method of probits. Science 79: 38–39. doi: 10.1126/science.79.2037.38

Borchert, D.M., Walgenbach, J.F., Kennedy, G.G., and L Long, J.W. 2004. Toxicity and residual activity of methoxyfenozide and tebufenozide to codling moth (Lepidoptera: Tortricidae) and oriental fruit moth (Lepidoptera: Tortricidae). Journal of Economic Entomology 97: 1342–1352. doi: 10.1603/0022-0493-97.4.1342

Carlson, G.R., Dhadialla, T.S., Hunter, R R., Jansson, R R.K., Jany, C.S., L Lidert, Z., and Slawecki, R R.A. 2001. The chemical and biological properties of methoxyfenozide, a new insecticidal ecdysteroid agonist. Pest Management Science 57: 115–119. doi: 10.1002/1526-4998(200102)57:2<115::AI D-PS245>3.0.CO;2-A

Charmillot, P.J., Gourmelon, A A., Fabre, A A.L., and Pasquier, D. 2001. Ovicidal and larvicidal effectiveness of several insect growth inhibitors and regulators on the codling moth Cydia pomonella L L. (Lep., Tortricidae). Journal of Applied Entomology 125: 147–153. doi: 10.1673/031.008.2901

Dallaire, R R., L Labrecque, A A., Marcotte, M., Bauce, É., and Delisle, J. 2004. The sublethal effects of tebufenozide on the precopulatory and copulatory activities of Choristoneura fumiferana and C. rosaceana. Entomologia Experimentalis et Applicata 112: 169–181. doi: 10.1111/j.0013-8703.2004.00193.x

Dedes, J. 2009. Biology of the whitemarked tussock moth (Orgyia leucostigma) [online]. Available from http://cfs.nrcan.gc.ca/publications/download-pdf/33039 [accessed July 26, 2017].

Dhadialla, T.S., Carlson, G.R., and L Le, D.P. 1998. N New insecticides with ecdysteroidal and juvenile hormone activity. A Annual R Review of Entomology 43: 545–569. doi:

Hu, W., Feng, Q., Palli, S.R., Krell, P.J., A Arif, B.M., and R Retnakaran, A A. 2001. The ABC transporter Pdr5p mediates the efflux of nonsteroidal ecdysone agonists in Saccharomyces cerevisiae. European Journal of Biochemistry 268: 3416–3422. doi: 10.1046/j.1432-1327.2001.02245.x

Knight, A A.L., Dunley, J.E., and Jansson, R R.K. 2001. Baseline monitoring of codling moth (Lepidoptera: Tortricidae) larval response to benzoylhydrazine insecticides. Journal of Economic Entomology 94: 264–270. doi: 10.1603/0022-0493-94.1.264

Moulton, J.K., Pepper, D.A., and Dennehy, T.J. 2000. Pro-active management of beet armyworm (Spodoptera exigua) resistance to the I IGRs, tebufenozide and methoxyfenozide [online]. A Available from http://ag.arizona.edu/pubs/crops/az1177/az1177-1l.pdf [accessed July 26, 2017].

Nakagawa, Y. 2005. N Nonsteroidal ecdysone agonists. Vitamins and Hormones 73: 131–173. doi: 10.1016/S0083-6729(05)73005-3

Retnakaran, A A., Gelbic, I I., Sundaram, M., Tomkins, W., L Ladd, T., Primavera, M., Feng, Q., A Arif, B., Palli, R R., and Krell, P. 2001. Mode of action of the ecdysone agonist tebufenozide (RH-5992), and an exclusion mechanism to explain resistance to it. Pest Management Science 57: 951-957. doi: 10.1002/ps.377

Retnakaran, A A., Krell, P., Feng, Q., and A Arif, B. 2003. Ecdysone agonists: mechanism and importance in controlling insect pests of agriculture and forestry. Archives of Insect Biochemistry and Physiology 54: 187–199. doi: 10.1002/arch.10116

Robertson, J.L., R Russell, R R.M., Preisler, H.K., and Savin, N N.E. 2007. Bioassays with arthropods, 2nd edition. CRC Press, Boca R aton, FL, U SA. Pp. 71–76.

Sáenz-de-Cabezón I Irigaray, F.J., Marco, V., Zalom, F.G., and Pérez-Morenom I I. 2005. Effects of methoxyfenozide on Lobesia botrana Den & Schiff (Lepidoptera: Tortricidae) egg, larval and adult stages. Pest Management Science 61: 1133–1137. doi: 10.1002/ps.1082

Smagghe, G., and Degheele, D. 1994. A Action of a novel nonsteroidal ecdysteroid mimic, tebufenozide (RH-5992), on insects of different orders. Pesticide Science 42: 85–92. doi: 10.1002/ps.2780420204

Smagghe, G., Carton, B., Heirman, A A., and Tirry, L L. 2000. Toxicity of four dibenzoylhydrazine correlates with evagination-induction in the cotton leafworm. Pesticide Biochemistry and Physiology 68: 49–58. doi: 10.1006/pest.2000.2497

Sundaram, M., Palli, S.R., I Ishaaya, I I., Krell, P.J., and R Retnakaran, A A. 1998. Toxicity of ecdysone agonists correlates with the induction of CHR3 mRNA RNA in the spruce budworm. Pesticide Biochemistry and Physiology 62: 201–208. doi: 10.1006/pest.1998.2383

Trisyono, A A., and Chippendale, G.M. 1998. Effect of the ecdysone agonists, R RH-2485 and tebufenozide, on the southwestern corn borer, Diatraea grandiosella. Pesticide Science 53: 177–185. doi: 10.1002/(SICI)1096-9063(199806)53:2<177::AI D-PS753>3.0.CO;2-6

van Frankenhuyzen, K.V., Gringorten, L L., Dedes, J., and Gauthier, D. 1997. Susceptibility of different instars of the spruce budworm (Lepidoptera: Tortricidae) to Bacillus thuringiensis var. kurstaki estimated with a droplet-feeding method. Journal of Economic Entomology 90: 560–565. doi: 10.1093/jee/90.2.560


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